ERYTHROCYTE METABOLISM. VI. SEPARATION OF ERYTHROCYTE ENZYMES FROM HEMOGLOBIN*
نویسندگان
چکیده
منابع مشابه
Separation of erythrocyte enzymes from hemoglobin by chromatography on Blue-Sepharose.
The spectrophotometric determination of the oxygen consumption of biological samples with Hb02 as oxygen donor and’indicator [ 1,2] has the advantage over conventional methods of being accurate, sensitive and suitable for automation [3,4]. Since interferences due to the erythrocyte enzymes are usually negligible, highly purified hemoglobin preparations are not generally required [5-81. However,...
متن کاملErythrocyte Metabolism
Previous studies from this laboratory on the preservation of erythrocytes stored in vitro have shown that the storage lesion, as characterized by the decline in level of organic phosphate esters, may be reversed by incubating the aged cells with certain purine nucleosides (1). Pyrimidine nucleosides were ineffective in this respect, as was the addition of the purine bases and ribose, alone or i...
متن کاملMaximal erythrocyte and hemoglobin catabolism.
A series of experiments were performed on anesthetized dogs in which varying quantities of normal canine erythrocytes damaged by incubation with N-ethylmaleimide were injected into the circulation. Cell sequestration and catabolism of hemoglobin to carbon monoxide remained normal after injections that contained from 0.058 to 0.154 g of hemoglobin per kg of body weight. After administration of l...
متن کاملEnzymes of the Human Erythrocyte
The enzymatic interconversion involves a net transfer of hydrogen which migrates as a proton (1). In spite of an early reference (2) to the role and existence of this glycolytic enzyme within a variety of animal tissues, it apparently has not been prepared previously in a significantly purified state from any source. Fractions rich in this enzyme have been separated from rabbit muscle (3), and ...
متن کاملEnzymes of the Human Erythrocyte
A detailed description of appropriate procedures for the isolation in presumably ahnost pure form of a purine nucleoside phosphorylase from human erythrocytes was presented as the original report of Paper I (1). The present paper will s ummarize the results of investigations concerning the stability characteristics and kinetic properties of the isolated enzyme. Enzymatic degradation of nucleosi...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1962
ISSN: 0021-9738
DOI: 10.1172/jci104588